Figure 1: AGEs activated ERK and P38 MAPK-NF-κB signal pathway by RAGE in HUVECs. (a-c) Effects of the AGEs and AGEs + anti-RAGE on ERK1/2 (a), p38 (b), and NF-κB (c) proteins in HUVECs. The HUVECs were treated with 200 mg/L HSA, 200 mg/L AGEs, and 50 mg/L anti-RAGE + 200 mg/L AGEs. ERK1/2, pERK1/2, p38, pp38, NF-κB p65, and pp65 proteins were detected by Western blotting. (d-f) Densitometry analysis of pERK1/2, pp38, and NF-κB pp65 is shown. AGEs: Advanced glycation end-products, RAGE: Receptor for advanced glycation end-products, HUVECs: Human umbilical venous endothelial cells, NF-κB: Nuclear factor-kappa B, MAPK: Mitogen-activated protein kinase