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  Indian J Med Microbiol
 

Figure 5: Arterial patches lose Ephrin-B2 and gain Eph-B4 expression in the rat inferior vena cava. (a-c) Bar graphs show relative mRNA transcript expression of Eph-B4 and Ephrin-B2 after direct suture (a), jugular vein patch (b), or carotid artery patch (c), day 0 and day 14; *P <0.05 (t-test); n = 3. (d) Representative Western blot showing Ephrin-B2, Eph-B4, and GAPDH expression after direct suture, jugular vein patch, or carotid artery patch, day 0 and day 14; n = 3. (e-f) Bar graphs show densitometry of Eph-B4 (e) and Ephrin-B2 (f) protein expression after direct suture, jugular vein patch, or carotid artery patch, day 0 and day 14; *P <0.005 versus day 0 (t-test); n = 3. (g) Immunofluorescence of the neointima in native inferior vena cava, carotid artery, day 0; and after direct suture, jugular vein patch, carotid artery patch, day 14. First row, merge of Eph-B4(red) and Ephrin-B2 (green); second row, merge of Eph-B4 (green) and vWF (red); third row, merge of Eph-B4 (green) and α-actin (red); DAPI, blue; L, lumen; scale bar, 100 μm; n = 5. (h) Bar graph showing Eph-B4density in the endothelial cells, day 14; *P <0.005 (ANOVA); n = 5

Figure 5: Arterial patches lose Ephrin-B2 and gain Eph-B4 expression in the rat inferior vena cava. (a-c) Bar graphs show relative mRNA transcript expression of Eph-B4 and Ephrin-B2 after direct suture (a), jugular vein patch (b), or carotid artery patch (c), day 0 and day 14; *<i>P</i> <0.05 (<i>t</i>-test); <i>n</i> = 3. (d) Representative Western blot showing Ephrin-B2, Eph-B4, and GAPDH expression after direct suture, jugular vein patch, or carotid artery patch, day 0 and day 14; <i>n</i> = 3. (e-f) Bar graphs show densitometry of Eph-B4 (e) and Ephrin-B2 (f) protein expression after direct suture, jugular vein patch, or carotid artery patch, day 0 and day 14; *<i>P</i> <0.005 versus day 0 (<i>t</i>-test); <i>n</i> = 3. (g) Immunofluorescence of the neointima in native inferior vena cava, carotid artery, day 0; and after direct suture, jugular vein patch, carotid artery patch, day 14. First row, merge of Eph-B4(red) and Ephrin-B2 (green); second row, merge of Eph-B4 (green) and vWF (red); third row, merge of Eph-B4 (green) and α-actin (red); DAPI, blue; L, lumen; scale bar, 100 μm; <i>n</i> = 5. (h) Bar graph showing Eph-B4density in the endothelial cells, day 14; *<i>P</i> <0.005 (ANOVA); <i>n</i> = 5