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Figure 1: Closure of rat aorta. (a) Representative photomicrographs of the rat aorta closed with direct suture, jugular vein patch, or carotid artery patch, day 0 and day 14; first row (low power, elastin van Gieson staining), scale bar, 1 mm; second row (high power, elastin van Gieson staining); third row (high power, trichrome Masson staining); fourth row (high power, αactin); scale bar, 100 μm. L, lumen; N, neointima; J, jugular vein patch; C, carotid artery patch; day 0, n = 3, day 14, n = 5. (b) Bar graph showing neointimal thickness, day 14; P = 0.011 (ANOVA); n = 3–5. (c) Bar graph showing patch thickness, day 0 and day 14; *P < 0.05, versus day 0 (ttest); n = 3–5. (d) Bar graph showing the luminal area of the aorta, day 0 and day 14; P = 0.0258 (ttest); n = 3–5. (e) Immunofluorescence of the neointima after direct suture, jugular vein patch, carotid artery patch, day 14. First row, merge of CD34 (green) and VEGFR2 (red); second row, merge of CD34 (green) and EphrinB2 (red); third row, merge of CD34 (green) and EphB4 (red); DAPI, blue; L, lumen; scale bar, 100 μm; n = 3–5. (f) Bar graph showing the percentage of CD34/EphrinB2dualpositive or CD34/EphB4dualpositive cells among all CD34positive neointimal cells, day 14; n = 3–5 
